Are hexokinase and glucokinase isozymes?

Last Update: May 30, 2022

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Asked by: Kailee Grady
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Glucokinase (hexokinase D) is a monomeric cytoplasmic enzyme found in the liver and pancreas that serves to regulate glucose levels in these organs. ... Glucokinase is a hexokinase isoenzyme.

What is the difference between glucokinase and hexokinase?

The main difference between hexokinase and glucokinase is that the hexokinase is an enzyme present in all cells whereas the glucokinase is an enzyme only present in the liver. Furthermore, hexokinase has a high affinity towards glucose while glucokinase has a low affinity towards glucose.

Is hexokinase phosphorylated?

A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. ... All hexokinases are capable of phosphorylating several hexoses but glucokinase acts with a 50-fold lower substrate affinity and its main hexose substrate is glucose.

Is hexokinase hydrophobic?


Hexokinases are cytosolic enzymes, but hexokinases I and II bind to mitochondria through a N-terminal hydrophobic region that interacts with voltage-dependent anion channel (VDAC). ... Furthermore, binding of hexokinase I to mitochondria decreases its affinity for the inhibitor glucose 6-phosphate.

Is hexokinase regulated?

Hexokinase, which catalyzes the entry of free glucose into the glycolytic pathway, is another regulatory enzyme.

Hexokinase vs. Glucokinase

23 related questions found

What regulates hexokinase?

Hexokinase, the enzyme catalyzing the first step of glycolysis, is inhibited by its product, glucose 6-phosphate. ... In turn, the level of glucose 6-phosphate rises because it is in equilibrium with fructose 6-phosphate. Hence, the inhibition of phosphofructokinase leads to the inhibition of hexokinase.

What is the main function of hexokinase?

Hexokinase is the initial enzyme of glycolysis, catalyzing the phosphorylation of glucose by ATP to glucose-6-P. It is one of the rate-limiting enzymes of glycolysis. Its activity declines rapidly as normal red cells age.

Is glucokinase a hexokinase?

Glucokinase (GK) is a hexokinase isozyme, related homologously to at least three other hexokinases. All of the hexokinases can mediate phosphorylation of glucose to glucose-6-phosphate (G6P), which is the first step of both glycogen synthesis and glycolysis.

What type of enzyme is hexokinase?

A hexokinase is an enzyme that phosphorylates hexoses, which are sugars with six carbons, resulting in hexose phosphate. Glucoseis is the most important substrate of hexokinases in most organisms, while glucose-6-phosphate is the most important product.

Which hormone is responsible for Glycogenolysis?

Glucagon promotes glycogenolysis in liver cells, its primary target with respect to raising circulating glucose levels.

What does hexokinase bind to?

Hexokinase is the enzyme that catalyzes this phosphoryl-group-transfer. Hexokinase undergoes and induced-fit conformational change when it binds to glucose, which ultimately prevents the hydrolysis of ATP. It is also allosterically inhibited by physiological concentrations of its immediate product, glucose-6-phosphate.

Where is hexokinase III found?

HK3 is the predominant hexokinase in myeloid cells, particularly granulocytes.

Is glucokinase in the liver?

Glucokinase is expressed primarily in the liver and in the pancreatic β-cell. In the liver, it is involved in glycogenesis in response to postprandial elevations in glucose.

What is glucokinase inhibited by?

Glucokinase is inhibited in the postabsorptive state by sequestration in the nucleus bound to GKRP, and it is activated postprandially by portal hyperglycemia and fructose through dissociation from GKRP, translocation to the cytoplasm, and binding to PFK2/FBP2.

What are the 10 steps in glycolysis?

Glycolysis Explained in 10 Easy Steps
  • Step 1: Hexokinase. ...
  • Step 2: Phosphoglucose Isomerase. ...
  • Step 3: Phosphofructokinase. ...
  • Step 4: Aldolase. ...
  • Step 5: Triosephosphate isomerase. ...
  • Step 6: Glyceraldehyde-3-phosphate Dehydrogenase. ...
  • Step 7: Phosphoglycerate Kinase. ...
  • Step 8: Phosphoglycerate Mutase.

Is hexokinase activated by insulin?

Insulin has several effects in liver which stimulate glycogen synthesis. First, it activates the enzyme hexokinase, which phosphorylates glucose, trapping it within the cell.

Does ATP bind to hexokinase?

Mechanism of Hexokinase

To start this reaction, ATP forms a complex with magnesium (II) ion and glucose binds to hexokinase. The magnesium-ATP complex then binds with the hexokinase-glucose complex and forms an intermediate (Zeng, et al.

What is the function of glucokinase?

Glucokinase (GCK) is a gene which plays an important role in recognising how high the blood glucose is in the body. It acts as the “glucose sensor” for the pancreas, so that when the blood glucose rises, the amount of insulin produced also increases.

What is hexokinase test?

A highly specific method for determining the concentration of glucose in serum or plasma by spectrophotometrically measuring the NADP formed from hexokinase-catalyzed transformations of glucose and various intermediates.

What is meant by glucokinase?

: a hexokinase found especially in the liver that catalyzes the phosphorylation of glucose.

Do muscle cells have glucokinase?

To increase glucose phosphorylation, the hepatic glucose-phosphorylating enzyme glucokinase has also been expressed in skeletal muscle (17).

What is a glucokinase activator?

Glucokinase activators (GKAs) have been discovered recently that stimulate the enzyme allosterically by lowering its glucose S0.5 (the concentration of glucose that allows half-maximal activity of the enzyme) and Hill coefficient (nH) and increasing its catalytic constant (kcat).

What happens if hexokinase is stopped?

Hexokinase's role in ATP production

Because hexokinase is involved in this preliminary step, if its activity were stopped, the cell could not continue to produce ATP by either pathway.

What is hexokinase made up of?

Hexokinase is a large homodimer composed of 920 amino acids in each chain. ... Hexokinase is capable of binding two ligands, glucose and glucose-6-phosphate. Glucose is bound so that glycolysis can occur, and glucose-6-phosphate is bound as an allosteric inhibitor . It may also be helpful to view this structure in stereo .

Why is hexokinase not in the liver?

Liver contains an enzyme called glucokinase, which also carries out the [hexokinase] reaction, but is highly specific for D-glucose, has a much higher Km for glucose (approximately 10.0 mM ), and is not product-inhibited... ... It is weakly inhibited by glucose 6-phosphate (with an inhibition constant around 50 mM ).